Regulatory

Part:BBa_K1913026:Design

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)


Wild type ptet-FixK2 hybrid promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to make Fixk2 promoter as an inducible promoter for the toggle switch, we had to add additional repressor operator sequences into the Fixk2 sequence, they are both inserted in the core element region of the Fixk2 sequence, which could be bonded by dimerized TetR repressors resulting in the formation of steric hindrance and consequently on transcription repression.


Source

Genomic sequence of Bradyrhizobium japonicum

References